The Ultimate Guide To Amorphispironone
The Ultimate Guide To Amorphispironone
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Amorphispironone (Amorphispironon E) is definitely an ichthysanoid isolated from Amorpha fruticosa that exhibits substantial anti-tumor selling results on skin tumors in mice and can be used inside the examine of tumors.
Get amorpha fruticosa leaf,, acquire by weighing 1kg, join supercritical CO as a result of pulverization process 2In the extractor, as entrainment agent, usage is eighteen% of the medicinal materials bodyweight with ethanol, extracts, and extraction temperature is 30 ℃, and extracting strain is 32MPa, CO 2Flow is 4ml/g crude drug/min, and the extraction time is 3.5h, and separating however I temperature is thirty ℃, and stress is 8MPa, and separating continue to II temperature is 28 ℃, and stress is 7MPa, obtains extract, stirs with ETHYLE ACETATE and would make dissolving, filters, as well as filtrating concentrate drying gets crude extract; Adopt the Phony indigo spiral shell ketone while in the substantial-velocity countercurrent chromatography separation and purification crude extract, its two-section solvent program is petroleum ether-ethyl acetate-methanol-drinking water, and volume ratio is 6:1:5:one, on to fill Using the significant velocity adverse current chromatogram article mutually be stationary section; Rotate most important body, pump into down and do moving phase mutually, shifting stage is dissolved crude extract with the sampling valve sample introduction, the UV-detector on-line checking; The detection wavelength is 209nm, collects Wrong indigo spiral shell ketone component, vacuum concentration; Separate out crystallization, leach crystallization, add the dissolving of 80% methanol eddy; Location recrystallization, filtration, washing are drying to acquire Bogus indigo spiral shell ketone, material 97.4%. Embodiment three:
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(three) the Fake indigo spiral shell ketone within the utilizing substantial-speed countercurrent chromatography separation and Amorphispironon E purification crude extract; Its two-period solvent technique is petroleum ether-ethyl acetate-methanol-water, on to fill While using the substantial pace adverse current chromatogram write-up mutually be stationary stage, rotate main frame; Pump into down and do moving stage mutually; Moving phase dissolving crude extract is via the sampling valve sample introduction, along with the UV-detector on-line checking is collected Bogus indigo spiral shell ketone component;
2006). PCA assessed the conformational dynamics of unliganded ITK and its complexes with 3 bioactive ligands: Withanolide A, Amorphispironon E, and 27-DHA. Structural sampling was carried out by analyzing trajectories of C
Framework and stereochemistry of amorphispironone, a novel cytotoxic spironone type rotenoid Amorphispironon E from Amorpha fruticosa
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混匀澄清 以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
An appropriate ITK composition was recognized and retrieved from UniProt and PDB based on significant resolution, the existence of the kinase domain, a mutation sequence, an affiliated ligand, and the quality of the construction.